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Re: Roadster (844 Views)
Posted by: Blueskies (IP Logged)
Date: April 25, 2019 12:13PM

Here you go-
The half life of Lasix is around 2 hours so it's not around long.


Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix) in equine blood by a one step ELISA and PCFIA.
Woods WE1, Wang CJ, Houtz PK, Tai HH, Wood T, Weckman TJ, Yang JM, Chang SL, Blake JW, Tobin T, et al.
Author information
Abstract
A one step enzyme-linked immunosorbent assay (ELISA) and a particle concentration fluorescent immunoassay (PCFIA) test for furosemide were evaluated as part of a panel of pre- and post-race tests for illegal medication of racing horses. These tests are very sensitive to furosemide with an I-50 for furosemide of about 20 ng/ml. The test is also rapid; an average pre-race complement of 10 samples can be analyzed in 90 minutes or less. The ELISA test results can be read with an inexpensive spectrophotometer, or even by eye. Both the PCFIA test and the ELISA test readily detect the presence of furosemide in equine blood for up to five hours after administration of the recommended therapeutic dose of this agent. The principal utility of these tests lies in rapid screening of samples for compliance with regulations governing the use of furosemide. Thus these tests can be used pre-race to determine whether horsemen have treated their horses with furosemide, and post-race to perform an initial evaluation of whether certain blood concentrations of furosemide have been exceeded. Pilot trials with these systems in Kentucky and Illinois suggest that these tests are economical and effective, and can form part of an analytical approach to substitute for the detention barn system of monitoring furosemide administration.



Subject Written By Posted
Roadster (1370 Views) wrongly1 04/24/2019 11:03AM
Re: Roadster (1072 Views) dglass2232 04/24/2019 11:54AM
Re: Roadster (1008 Views) wrongly1 04/24/2019 06:44PM
Re: Roadster (880 Views) Roman 04/24/2019 11:18PM
Re: Roadster (930 Views) Roman 04/24/2019 11:32PM
Re: Roadster (842 Views) Blueskies 04/25/2019 07:57AM
Re: Roadster (877 Views) Niall 04/25/2019 08:26AM
Re: Roadster (920 Views) TGJB 04/25/2019 09:29AM
Re: Roadster (884 Views) JimP 04/25/2019 09:48AM
Re: Roadster (890 Views) TGJB 04/25/2019 10:33AM
Re: Roadster (844 Views) Blueskies 04/25/2019 12:13PM
Re: Roadster (837 Views) TGJB 04/25/2019 12:26PM
Re: Roadster (815 Views) TGJB 04/25/2019 04:06PM
Re: Serengeti Empress (870 Views) BitPlayer 04/25/2019 12:12PM


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